Stefano Lorenzetti (SL) is a staff researcher at the Dpt. of Food Safety and Veterinary Public Health of the Italian National Health Institute (Istituto Superiore di Sanità - ISS) in Rome.
SL graduated in Biological Sciences (1991) and in Human Nutrition Sciences (2003) and appointed as a member of the Italian national expert group of “Alternative methods to animal experimentation” (since 2012).
SL research is focused on the set up of in vitro alternative methods to animal experimentation to study the role of endocrine disruptors/EDCs or Endocrine Active Substances/EASs such as bioactive compounds of plant origin (e.g. polyphenols) and environmental and dietary contaminants (e.g., plasticizers, pesticides and biocides).
SL participated to different international and national projects on the hazard and risk characterization of environmental and dietary contaminants as well as on the characterization of biomarkers to be used in the screening of hormone-dependent male diseases and EDC-like adverse effects, such as: i) the EU-granted LIFE-EDESIA project (“Endocrine Disruptors in silico / in vitro - Evaluation and Substitution for Industrial Applications”; LIFE12 ENV/IT/000633; 2013-2016); ii) the EU Integrated Project ReProTect (“Development of a novel approach in hazard and risk assessment or reproductive toxicity by a combination and application of in vitro, tissue and sensor technologies”; 2006-2010) to develop an integrated in vitro approach linking toxicogenomics to clinical biomarkers (phenotypic anchoring); iii) the NIH-ISS project “Tackling rare diseases yet lacking diagnosis and/or prognosis: a pilot project integrating data collection and experimental studies” (2007-10) linking in utero DEHP exposure to liver programming and hepatoblastoma; iv) the national project PRO-HEALTH-Zinc (“Biomonitoring of zinc and selenium as biomarkers of clinical support to PSA measurement for the prediction of prostate diseases“; in progress); and v) the Italian Health Ministery granted project “In vitro alternative methods: male reproductive prostate-mediated toxicity of potential SVHC “ (2012-13).
- Verze P, Cai T, Lorenzetti S. 2016. Male fertility and the role of the prostate in health and disease. Nature Reviews Urology, published online 01.06.2016, doi: 10.1038/nrurol.2016.89
- Lorenzetti S, Marcoccia D, Mantovani A. 2015. Biomarkers of Effect in Endocrine Disruption: How to link a Functional Assay to an Adverse Outcome Pathway. Annals Ist Super Sanità, 51(2):167-171.
- Roncaglioni A, Benfenati E, Mantovani A, Fiorino F, Perissutti E, Lorenzetti S. 2015. The role of in silico tools in supporting the application of the substitution principle. ALTEX, 32(2):151-3.
- Smeriglio A, Trombetta D, Marcoccia D, Narciso L, Mantovani A, Lorenzetti S. 2014. Intracellular distribution and biological effects of plant bioactives in a sex steroid-sensitive model of human prostate adenocarcinoma. Anti-cancer Agents Med Chem., 14(10):1386-96.
- Marcoccia D, Georgiev MI, Alipieva K, Lorenzetti S. 2014. Inhibition of the DHT-induced PSA secretion by Verbascum xanthophoeniceum and Serenoa repens extracts in human LNCaP prostate epithelial cells. Ethnopharmacol., 155(1):616-25.
- Lorenzetti S, Mantovani A. 2014. Reproductive and Developmental Toxicity Testing: issues for 3Rs implementation. In: Reducing, Refining, and Replacing the Use of Animals in Toxicity Testing (Chapter 12, pp. 330-347), edited by Dave G Allen and Michael D Waters, RSC Publishing, Cambridge (UK); DOI:10.1039/9781849737920-00330.
- Rovida C, De Angelis I, Lorenzetti S. 2013. Alternative in vitro methods to characterize the role of Endocrine Active Substances (EASs) in hormone-targeted tissues. ALTEX, 30(2):253-5.
- Lorenzetti S, Narciso L. 2012. Nuclear receptors: connecting human health to the environment. In: Computational approaches to nuclear receptors (Chapter 1, pp. 1-22), edited by Pietro Cozzini and Glen E. Kellogg, Drug Discovery series of Royal Society of Chemistry, RSC Publishing, Cambridge (UK); DOI:10.1039/9781849735353-00001.
- Lorenzetti S, Marcoccia D, Narciso L, Mantovani A. 2010. Cell viability and PSA secretion assays in LNCaP cells: a tiered in vitro approach to screen chemicals with a prostate-mediated effect on male reproduction within the ReProTect project. Reprod Toxicol, 30(1):25-35.